Theoretical development and experimental evaluation of imaging models for differential-interference-contrast microscopy.
نویسندگان
چکیده
Imaging models for differential-interference-contrast (DIC) microscopy are presented. Two- and three-dimensional models for DIC imaging under partially coherent illumination were derived and tested by using phantom specimens viewed with several conventional DIC microscopes and quasi-monochromatic light. DIC images recorded with a CCD camera were compared with model predictions that were generated by using theoretical point-spread functions, computer-generated phantoms, and estimated imaging parameters such as bias and shear. Results show quantitative and qualitative agreement between model and data for several imaging conditions.
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عنوان ژورنال:
- Journal of the Optical Society of America. A, Optics, image science, and vision
دوره 16 9 شماره
صفحات -
تاریخ انتشار 1999